Journal: Journal of vascular research

Article Title: Transplantation of autologous bone marrow mononuclear cells regulates inflammation in a rabbit model of carotid artery atherosclerosis

doi: 10.1159/000449201

Figure Lengend Snippet: BMMNCs treatment downregulated pro-inflammatory mediators IL-6 and CD147 but increased the production of anti-inflammatory cytokines IL-10 and TGF-β. (A) RT-PCR and Western blot (F) analysis of IL-6, IL-10, eNOS and TGF-β on days 1, 3, 7 and 14 after BMMNCs injection. (B–E) Quantification analysis of RT-PCR bands showed BMMNCs treatment reduced the expression of IL-6 and CD147 but increased the production of IL-10 and TGF-β; (G–J) Western blot results were consistent with RT-PCR assay *p<0.05 vs corresponding vehicle groups. n=6/group.

Article Snippet: Then incubated with primary antibody as follows: rabbit anti-rabbit CD147 (1:400, BIOSS, Beijing, China), cavia anti-rabbit IL-6 (1:400, Uscn Life science Inc, Wuhan, China), mouse anti-rabbit IL-10 (1:500, Global Biotech, Shanghai, China), rabbit anti-rabbit TGF-β1 (1:500, Global Biotech), mouse anti-rabbit β-Actin (1:500, BOSTER) for 2h at room temperature or overnight at 4 °C.

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Injection, Expressing

Journal: Oncology Letters

Article Title: Expression and clinical significance of extracellular matrix metalloproteinase inducer, EMMPRIN/CD147, in human osteosarcoma

doi: 10.3892/ol.2012.981

Figure Lengend Snippet: Correlation between EMMPRIN/CD147 expression and clinicopathological characteristics.

Article Snippet: The sections were blocked with 5% bovine serum albumin (Zhongshan, Beijing, China) in PBS solution for 20 min and probed with rabbit anti-human EMMPRIN/CD147 antibodies (1:300; Boster) at 4°C for 12 h. After washing, the bound antibodies were detected with biotinylated goat anti-rabbit IgG (1:100) and SABC complex at 30°C for 20 min.

Techniques: Expressing

Journal: iScience

Article Title: Glutamate-specific gene linked to human brain evolution enhances synaptic plasticity and cognitive processes

doi: 10.1016/j.isci.2024.108821

Figure Lengend Snippet:

Article Snippet: Rabbit Polyclonal anti-GDH1 , AVIVA SYSTEM BIOLOGY , ARP45709_P050; RRID: AB_2046094.

Techniques: Plasmid Preparation, Immunostaining, Western Blot, Virus, Recombinant, Protease Inhibitor, Transfection, Software, Membrane

Journal: Cell & Bioscience

Article Title: Development of a novel human CD147 knock-in NSG mouse model to test SARS-CoV-2 viral infection

doi: 10.1186/s13578-022-00822-6

Figure Lengend Snippet: Schematic representation of genotyping primers used to confirm hCD147KI expression. A combination of 4 primers was used to screen mice (internal primers hCD147A and hCD147B) and confirm proper integration into the mouse CD147 allele (flanking primers BSGC and BSGD)

Article Snippet: To stain mouse CD147 (mCD147), polyclonal donkey anti-goat IgG secondary (Jackson ImmunoResearch; 705–035-003 [HRP]; 1:500) was used against primary goat anti-mouse CD147 antibody (R&D Systems; BAF772; 1:100).

Techniques: Expressing

Journal: Cell & Bioscience

Article Title: Development of a novel human CD147 knock-in NSG mouse model to test SARS-CoV-2 viral infection

doi: 10.1186/s13578-022-00822-6

Figure Lengend Snippet: H&E and IHC of human CD147 in hCD147KI het -NSG mice. Human CD147 was stained (HIM6; 1:500) in the ( A ) lung, ( B ) liver, ( C ) intestine, ( D ) heart, ( E ) brain, ( F ) spleen, ( G ) kidney, ( H ) testis, and ( I ) trachea in WT-NSG (top) and hCD147KI het -NSG (bottom) mice. Images were taken using an Olympus Inverted Light Microscope. Scale bar represents 100 µm

Article Snippet: To stain mouse CD147 (mCD147), polyclonal donkey anti-goat IgG secondary (Jackson ImmunoResearch; 705–035-003 [HRP]; 1:500) was used against primary goat anti-mouse CD147 antibody (R&D Systems; BAF772; 1:100).

Techniques: Staining, Light Microscopy

Journal: Cell & Bioscience

Article Title: Development of a novel human CD147 knock-in NSG mouse model to test SARS-CoV-2 viral infection

doi: 10.1186/s13578-022-00822-6

Figure Lengend Snippet: Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KI het -NSG (bottom) cells from ( A ) PBMCs, ( B ) lung, ( C ) liver, and ( D ) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining

Article Snippet: To stain mouse CD147 (mCD147), polyclonal donkey anti-goat IgG secondary (Jackson ImmunoResearch; 705–035-003 [HRP]; 1:500) was used against primary goat anti-mouse CD147 antibody (R&D Systems; BAF772; 1:100).

Techniques: Expressing, Staining

Journal: Cell & Bioscience

Article Title: Development of a novel human CD147 knock-in NSG mouse model to test SARS-CoV-2 viral infection

doi: 10.1186/s13578-022-00822-6

Figure Lengend Snippet: Diagram of proposed working hypothesis of CD147 in SARS-CoV-2 infection. (1) SARS-CoV-2 virions infect human cells via the canonical pathway where host Angiotensin-converting Enzyme 2 (ACE2) receptors bind to viral spike proteins (red) and facilitate viral entry and infection. (2) CD147 proteins, via binding to surface binding partners (e.g., E-selectin), facilitate cell–cell adhesion, membrane fusion, and intercellular transfer of SARS-CoV-2 virions. (3) Erythrocytes and platelets which strongly express CD147, bind SARS-CoV-2 virions, and increase thrombosis risk and other clinical manifestations of COVID-19

Article Snippet: To stain mouse CD147 (mCD147), polyclonal donkey anti-goat IgG secondary (Jackson ImmunoResearch; 705–035-003 [HRP]; 1:500) was used against primary goat anti-mouse CD147 antibody (R&D Systems; BAF772; 1:100).

Techniques: Infection, Binding Assay, Membrane

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Extracellular matrix metalloproteinase inducer regulates metalloproteinases in human uterine endometrium.

doi: 10.1210/jc.2005-0601

Figure Lengend Snippet: FIG. 1. EMMPRIN protein localization in cycling human eutopic uter- ine endometrium. Tissue sections were probed with human anti- EMMPRIN antibody or nonspecific IgG. The stages of the menstrual cycle shown are: A, early menses; control, nonspecific IgG; B–G, probed with anti-EMMPRIN antibody; B, early menses (inset, 40); C, early proliferative (inset, 40); D, midproliferative (20); E, early secretory (inset luminal epithelium, 40); F, midsecretory; and G, late secretory (inset luminal epithelium; 40). Unless noted, all pictures were taken at 20 magnification. Arrowheads denote EMMPRIN localization to lu- minal epithelial cells. s, Stroma; l, uterine lumen; g, gland.

Article Snippet: Slides were then incubated with 2 g/ml goat polyclonal antibody against recombinant human EMMPRIN (R&D Systems, Inc.) in 1% BSA in PBS at 4 C overnight.

Techniques: Control

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Extracellular matrix metalloproteinase inducer regulates metalloproteinases in human uterine endometrium.

doi: 10.1210/jc.2005-0601

Figure Lengend Snippet: FIG. 3. Changes in MMP-1, -2, and -3 and EMMPRIN mRNA levels in response to treatment with EMMPRIN or IL-1. RNA was collected after treatment of HUF cells with either IL-1 or EMMPRIN at the indicated concentrations for 24 h. Fold changes in mRNA levels were quantitated by TaqMan PCR normalized to GAPDH and calibrated to control levels. A, Changes in MMP-1 and -2 mRNA levels; B, changes in MMP-3 mRNA levels; C, changes in EMMPRIN mRNA levels (n 3; P 0.05). Statistical significance was calculated from normalized data and is indicated by different letters above treatments.

Article Snippet: Slides were then incubated with 2 g/ml goat polyclonal antibody against recombinant human EMMPRIN (R&D Systems, Inc.) in 1% BSA in PBS at 4 C overnight.

Techniques: Control

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Extracellular matrix metalloproteinase inducer regulates metalloproteinases in human uterine endometrium.

doi: 10.1210/jc.2005-0601

Figure Lengend Snippet: FIG. 2. Changes in EMMPRIN mRNA levels in uterine endometrium throughout the menstrual cycle. Biopsies of eutopic endometrium from throughout the menstrual cycle were analyzed by real-time PCR for EMMPRIN mRNA expression. EMMPRIN mRNA levels were normalized to GAPDH and calibrated to the late secretory stage to determine relative expression levels. Bar A indicates significant dif- ferences between proliferative and secretory stages, bar B indicates significant differences between mid- and late secretory stages, and bar C indicates significant differences between early and late secre- tory stages (n 4 for each cycle stage; P 0.05).

Article Snippet: Slides were then incubated with 2 g/ml goat polyclonal antibody against recombinant human EMMPRIN (R&D Systems, Inc.) in 1% BSA in PBS at 4 C overnight.

Techniques: Real-time Polymerase Chain Reaction, Expressing

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Extracellular matrix metalloproteinase inducer regulates metalloproteinases in human uterine endometrium.

doi: 10.1210/jc.2005-0601

Figure Lengend Snippet: FIG. 4. Changes in MMP-1, -2, and-3 protein secretion in response to EMMPRIN or IL-1. A, Immunoblots of conditioned medium samples collected from treated HUF cells. Membranes were probed with spe- cific antibodies and detected with appropriate secondary antibodies. Lane 1, Control IL-1; lane 2, 10 ng/ml IL-1; lane 3, control EMMPRIN; lane 4, 0.5 g/ml EMMPRIN; lane 5, 1 g/ml EMMPRIN (n 3). Treatments of cells with IL-1 and EMMPRIN were con- ducted in separate experiments; therefore, two sets of untreated con- trol cells are shown. B, Densitometric analysis of immunoblots was normalized to the values for untreated control medium samples. Bars indicate the SE of treatments.

Article Snippet: Slides were then incubated with 2 g/ml goat polyclonal antibody against recombinant human EMMPRIN (R&D Systems, Inc.) in 1% BSA in PBS at 4 C overnight.

Techniques: Western Blot, Control

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Extracellular matrix metalloproteinase inducer regulates metalloproteinases in human uterine endometrium.

doi: 10.1210/jc.2005-0601

Figure Lengend Snippet: FIG. 5. EMMPRIN protein and mRNA expression in ectopic human endometrium (endometriotic lesions). A, Representative immunohis- tochemical analysis of EMMPRIN protein localization in lesions from three patients. Patient 1, a, Control, nonspecific IgG; c, anti- EMMPRIN antibody; patient 2, b, control, nonspecific IgG; d, anti- EMMPRIN antibody; e and f, patient 3, anti-EMMPRIN antibody. The arrowhead denotes EMMPRIN expression in peritoneal mesothe- lial cells. Magnification of photos: a and c, 20; b and d, 40; e, 20; and f, 40. ms, Mesothelial stroma; ge, uterine glandular epithelium; us, uterine stroma. B, Real-time PCR analysis of matched eutopic and ectopic endometria for EMMPRIN mRNA levels. Samples were nor- malized to endogenous control GAPDH mRNA levels and calibrated to eutopic endometrium of disease-free controls at the same stage of the menstrual cycle. The experimental unit is indicated above each sample.

Article Snippet: Slides were then incubated with 2 g/ml goat polyclonal antibody against recombinant human EMMPRIN (R&D Systems, Inc.) in 1% BSA in PBS at 4 C overnight.

Techniques: Expressing, Control, Real-time Polymerase Chain Reaction

Journal: BMC cancer

Article Title: CD73 complexes with emmprin to regulate MMP-2 production from co-cultured sarcoma cells and fibroblasts.

doi: 10.1186/s12885-019-6127-x

Figure Lengend Snippet: Fig. 1 MS analysis of emmprin complexes identified CD73 and CD99. Proteins forming complexes with emmprin were identified from cancer cells alone or from co-cultures of cancer cells and fibroblasts, and were analyzed by immunoprecipitation, cross-linking, and mass spectrometric (MS) protein identification. A total of 548 protein molecules were identified using MS. Overlap between proteins identified in different conditions (tumor cell only or three molecular weight regions under co-culture conditions #1–3) is shown. CD73 and CD99 identified in the overlap of all three co- culture conditions were selected for investigation of their effect on regulation of MMP-2 production

Article Snippet: Cell lysates (1 ml) were incubated with 2 μg anti-emmprin antibody (goat polyclonal antibody, R&D System), or anti-CD73 antibody (rabbit monoclonal, Cell Signaling) for 3 h. Protein G Sepharose (GE Healthcare, Chicago, Illinois) was used to immunoprecipitate proteins linked to the primary antibody overnight at 4 °C.

Techniques: Immunoprecipitation, Molecular Weight, Co-Culture Assay

Journal: BMC cancer

Article Title: CD73 complexes with emmprin to regulate MMP-2 production from co-cultured sarcoma cells and fibroblasts.

doi: 10.1186/s12885-019-6127-x

Figure Lengend Snippet: Fig. 2 CD99 and CD73 form complexes with emmprin. a Complex formation between emmprin and CD99 was identified by immunoprecipitation and immunoblotting, performed in co-cultures of tumor cells and fibroblasts. Arrow indicates emmprin-CD99 complex. b Complex formation between emmprin and CD73 was identified by immunoprecipitation and immunoblotting, performed in co-cultures of tumor cells and fibroblasts. Arrow indicates emmprin-CD73 complex.

Article Snippet: Cell lysates (1 ml) were incubated with 2 μg anti-emmprin antibody (goat polyclonal antibody, R&D System), or anti-CD73 antibody (rabbit monoclonal, Cell Signaling) for 3 h. Protein G Sepharose (GE Healthcare, Chicago, Illinois) was used to immunoprecipitate proteins linked to the primary antibody overnight at 4 °C.

Techniques: Immunoprecipitation, Western Blot

Journal: BMC cancer

Article Title: CD73 complexes with emmprin to regulate MMP-2 production from co-cultured sarcoma cells and fibroblasts.

doi: 10.1186/s12885-019-6127-x

Figure Lengend Snippet: Fig. 5 Colocalization of emmprin/CD73 detected by immunofluorescent staining and in situ proximity ligation assay. Cytoplasmic CD73 (green) expression was observed in fibroblasts, tumor cells and co-culture cells. Membranous emmprin expression (red) was observed in tumor cells and co-cultured cells. Nuclei were stained with DAPI (blue). CD73 and emmprin were colocalized in tumor cells (b) and co-cultured cells (c). The green arrow points to fibroblasts expresssing green florescence (CD73); The yellow arrow points to tumor cells expressed yellow florescence (emmprin and CD73) (c). CD73 siRNA treatment causes downregulation of CD73 cytoplasmic expression, although, membranous emmprin expression was retained in co-cultured cells (d). The fluorescent red spots observed using in situ proximity ligation assay (PLA), indicating protein- protein colocalization in cells, confirmed the interaction between CD73 and emmprin. The detected dimers (emmprin/CD73) are represented as red dots in co-cultured cells (g). In cells transfected with CD73 siRNA prior to in situ PLA for emmmprin-CD73 interaction, CD73 siRNA treatment caused downregulation of red dots (h). Immunofluorescent staining, IF (a-d); In situ proximity ligation assay, PLA (e-h); fibroblast only (a, e); tumor cell only (b, f); co-culture (c-d, g-h)

Article Snippet: Cell lysates (1 ml) were incubated with 2 μg anti-emmprin antibody (goat polyclonal antibody, R&D System), or anti-CD73 antibody (rabbit monoclonal, Cell Signaling) for 3 h. Protein G Sepharose (GE Healthcare, Chicago, Illinois) was used to immunoprecipitate proteins linked to the primary antibody overnight at 4 °C.

Techniques: Staining, In Situ, Proximity Ligation Assay, Expressing, Co-Culture Assay, Cell Culture, Transfection

Journal: BMC cancer

Article Title: CD73 complexes with emmprin to regulate MMP-2 production from co-cultured sarcoma cells and fibroblasts.

doi: 10.1186/s12885-019-6127-x

Figure Lengend Snippet: Fig. 7 Expression of CD73 and emmprin in epithelioid sarcoma. The hematoxylin and eosin (H&E) section shows proliferation of severely atypical polygonal cells with enlarged hyperchromatic nuclei, forming irregular nests, accompanied by fibroblastic cells and fibrous stroma (a). Immunohistochemical (b-c) and fluorescent immunohistochemical (d-f) expression of CD73 and emmprin in epithelioid sarcoma specimen was examined. Both tumor cells and surrounding stromal cells were positive for CD73 (b). Membranous emmprin expression was observed only in tumor cells (c). Cytoplasmic CD73 (green) expression was observed in fibroblasts and in tumor cells (e). Membranous emmprin expression (red) was observed in tumor cells (f). Marger of figures (e) and (f). The green arrow, indicates fibroblasts expressing green florescence (CD73); The Yellow arrow, indicates tumor cells expressing yellow florescence (emmprin and CD73) (d). CD73 and emmprin were colocalized in tumor cells (e). Nuclei were stained with DAPI (blue). Expression of CD73 and emmprin was examined immunohistochemically in a total of ten tumors. All tumors show similar expression pattern (Additional file 6: Table S1)

Article Snippet: Cell lysates (1 ml) were incubated with 2 μg anti-emmprin antibody (goat polyclonal antibody, R&D System), or anti-CD73 antibody (rabbit monoclonal, Cell Signaling) for 3 h. Protein G Sepharose (GE Healthcare, Chicago, Illinois) was used to immunoprecipitate proteins linked to the primary antibody overnight at 4 °C.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: BMC cancer

Article Title: CD73 complexes with emmprin to regulate MMP-2 production from co-cultured sarcoma cells and fibroblasts.

doi: 10.1186/s12885-019-6127-x

Figure Lengend Snippet: Fig. 8 A pictorial representation of the interaction between emmprin on tumor cells and CD73 on fibroblasts. Emmprin mainly exists on tumor cells, and CD73 exists both on tumor cells and fibroblasts. Emmprin forms a complex with CD73, and regulates MMP-2 production in co-cultures of tumor cells and fibroblasts. Pro- MMP-2 produced by fibroblasts is probably activated by MT1-MMP expressed on tumor cells

Article Snippet: Cell lysates (1 ml) were incubated with 2 μg anti-emmprin antibody (goat polyclonal antibody, R&D System), or anti-CD73 antibody (rabbit monoclonal, Cell Signaling) for 3 h. Protein G Sepharose (GE Healthcare, Chicago, Illinois) was used to immunoprecipitate proteins linked to the primary antibody overnight at 4 °C.

Techniques: Produced